EXTRACTION, PURIFICATION, AND CRYSTALLIZATION OF GTPASE ERA FROM STAPHYLOCOCCUS AUREUS

E. A. Klochkova; Клочкова Э. А.; D. R. Islamov; Исламов Д. Р.; A. D. Biktimirov; Биктимиров А. Д.; A. V. Rogachev; Рогачев А. В.; S. Z. Validov; Валидов Ш. З.; A. G. Bikmullin; Бикмуллин А. Г.; A. V. Simakin; Симакин А. В.; G. S. Peters; Петерс Г. С.; M. M. Yusupov; Юсупов М. М.; K. S. Usachev; Усачев К. С.

doi:10.31857/S0023476123010137

EXTRACTION, PURIFICATION, AND CRYSTALLIZATION OF GTPASE ERA FROM STAPHYLOCOCCUS AUREUS

Authors: Klochkova E.A.¹, Islamov D.R.², Biktimirov A.D.², Rogachev A.V.³^,4, Validov S.Z.², Bikmullin A.G.⁵, Simakin A.V.⁶, Peters G.S.⁷, Yusupov M.M.⁸, Usachev K.S.²
Affiliations:
1. Казанский (Приволжский) федеральныйKazan Federal University, Kazan, 420008 Russia университет
2. Kazan (Volga Region) Federal University, Kazan, 420008 Russia
3. Moscow Institute of Physics and Technology, Dolgoprudny, Moscow Region, 141701 Russia
4. Joint Institute for Nuclear Research, Dubna, Moscow Region, 141980 Russia
5. Kazan Federal University, Kazan, 420008 Russia
6. Prokhorov General Physics Institute, Russian Academy of Sciences, Moscow, 119991 Russia
7. National Research Centre “Kurchatov Institute,” Moscow, 123098 Russia
8. Institut de génétique et de biologie moléculaire et cellulaire, 67400 Illkirch-Graffenstaden, France
Issue: Vol 68, No 2 (2023)
Pages: 276-280
Section: STRUCTURE OF MACROMOLECULAR COMPOUNDS
URL: https://rjmseer.com/0023-4761/article/view/673508
DOI: https://doi.org/10.31857/S0023476123010137
EDN: https://elibrary.ru/GBATXG
ID: 673508

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Abstract

Protein crystal structure studies are an important tool for drug design. The growth of high-quality crystals suitable for X-ray diffraction is the limiting factor and the bottleneck in obtaining the structural data. Here we report the extraction, purification, and crystallization of the protein GTPase Era from the pathogenic bacterium Staphylococcus aureus. In bacterial cells, GTPase Era acts as a ribosome assembly factor. This enzyme is responsible for the cell growth and division. However, its structure is poorly understood. We obtained crystals of Staphylococcus aureus GTPase Era, which can be used in further structural studies by single-crystal X-ray diffraction analysis.

Keywords

PROTEIN CRYSTAL STRUCTURE, X-RAY DIFFRACTION

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