Obtaining and optimizing the mass spectrometric determination of glutathione persulfide
| Dublin Core | PKP Metadata Items | Metadata for this Document | |
| 1. | Title | Title of document | Obtaining and optimizing the mass spectrometric determination of glutathione persulfide |
| 2. | Creator | Author's name, affiliation, country | R. A. Ishkaeva; Kazan (Volga Region) Federal University; Russian Federation |
| 2. | Creator | Author's name, affiliation, country | L. V. Lopukhov; Kazan (Volga Region) Federal University; Russian Federation |
| 2. | Creator | Author's name, affiliation, country | I. S. Nizamov; Kazan (Volga Region) Federal University; Russian Federation |
| 2. | Creator | Author's name, affiliation, country | Т. I. Abdullin; Kazan (Volga Region) Federal University; Russian Federation |
| 3. | Subject | Discipline(s) | |
| 3. | Subject | Keyword(s) | glutathione persulfide; glutathione; hydrogen sulfide; N-ethylmaleimide; HPLC mass spectrometry |
| 4. | Description | Abstract | Glutathione persulfide (GSSH) is an important cell metabolite involved in redox regulation and a potential therapeutic agent. Due to the instability and lack of commercial GSSH standards, an urgent task is to develop methods for its in situ generation and quantification in various test systems. The paper optimizes the conditions for obtaining GSSH in the reaction of oxidized glutathione with sodium sulfide with the aid of fluorescent monitoring of released hydrogen sulfide. The reaction was performed in the presence of an excess of N-ethylmaleimide to derivatize the generated GSSH and reduced glutathione (GSH) in similar amounts of both derivatives. A technique for determining GSSH by the level of GSH in a model reaction by HPLC-mass spectrometry in the multiple reactions monitoring mode is described. The contribution of the GSH impurity in the oxidized glutathione solution to the determined amount of GSSH as well as the detection limit of GSSH in the reaction mixture have been established. The results are of interest for the preparation and mass spectrometric analysis of biologically significant persulfides using various derivatizing agents. |
| 5. | Publisher | Organizing agency, location | The Russian Academy of Sciences |
| 6. | Contributor | Sponsor(s) |
Russian Science Foundation (23-75-01143) |
| 7. | Date | (DD-MM-YYYY) | 15.11.2024 |
| 8. | Type | Status & genre | Peer-reviewed Article |
| 8. | Type | Type | Research Article |
| 9. | Format | File format | |
| 10. | Identifier | Uniform Resource Identifier | https://rjmseer.com/0044-4502/article/view/684633 |
| 10. | Identifier | Digital Object Identifier (DOI) | 10.31857/S0044450224110101 |
| 10. | Identifier | eLIBRARY Document Number (EDN) | swobts |
| 11. | Source | Title; vol., no. (year) | Zhurnal Analiticheskoi Khimii; Vol 79, No 11 (2024) |
| 12. | Language | English=en | ru |
| 13. | Relation | Supp. Files |
Fig. 1. Dependence of the SF7-AM fluorescence signal (λex = 490 nm) on Na2S concentration. Conditions: Tris-HCl buffer solution (pH 7.4), 5 μM SF7-AM, incubation for 15 min at 37°C. (12KB) Scheme 1. Structures of N-ethylmaleimide adducts with reduced glutathione (GS–NEM) and its persulphide (GSS–NEM) (Mi – mono-isotopic molecular weight). (26KB) Fig. 2. MS- (a) and MS/MS spectra (b) of negative ions in GS–NEM solution (d/s – number of ion detections per second). (a) The mass of the main peak coincides with the calculated value. (b) Fragmentation of the ion with m/z 431.01. (30KB) Fig. 3. MS- (a), MS/MS spectra for the ion with m/z 463.07 (b); MS/MS/MS spectrum for ions m/z 463.07–306.07 (c) of reaction mixture products GSSG + H2S + NEM at negative ionisation polarity. (47KB) Fig. 4. Chromatograms for selected MRM transitions of the GS–NEM reference sample (10 μM) (a) and the reaction mixture GSSG + H2S + NEM (dilution factor 1 : 100) (b). (23KB) |
| 14. | Coverage | Geo-spatial location, chronological period, research sample (gender, age, etc.) | |
| 15. | Rights | Copyright and permissions |
Copyright (c) 2024 Russian Academy of Sciences |