Abstract
A fundamental problem in narcology is to clarify the mechanisms of development of alcohol dependence (AD), in the pathogenesis of which disturbances in redox processes and inflammation play an important role. Identification of associations between biological and clinical parameters clarifies the molecular mechanisms of disease pathogenesis. The aim of the work was to study peripheral markers of oxidative stress (OS) in patients with AD in the early period of post-abstinence state and to identify their relationship with the clinical characteristics of the disease and inflammatory factors. The parameters of 84 male AD patients were determined; the average age was 44.3 ± 8.2 years. Clinical characteristics were analyzed: patient age, age of alcohol withdrawal syndrome (AWS) formation, duration of the disease, duration of AWS. OS markers were determined in blood plasma – protein oxidation products (carbonyl proteins, CP) using 2,4-dinitrophenylhydrazine; lipids (products reacting with thiobarbituric acid (TBA), TBA-RP); DNA (8-hydroxy-2′-deoxyguanosine, 8-OH-dG). Inflammatory mediators – proinflammatory cytokines (IFNγ, IL-1β, IL-6, IL-8, IL-17A, TNFα) were determined in blood serum. Control for biological studies – blood samples of 80 conditionally healthy men, average age 40.9 ± 9.6 years. In patients with AD, an increase in CP, TBA-RP and all cytokines was detected compared to controls (p < 0.001); the concentration of 8-OH-dG did not differ from the control. The following were found: a direct connection between TBA-RP and the duration of the disease; inverse relationship between CP and the age of formation and duration of AWS. CP had an inverse relationship with IL-6. Positive correlations were found between 8-OH-dG and IL-6, TBA-RP and IL-8, TBA-RP and TNFα. Thus, the early period of the post-withdrawal state in AD patients is characterized by pronounced OS and inflammation. The results obtained expand knowledge about the integrative contribution of OS and inflammation factors to the pathogenesis of AD and can be used in the development of new treatment methods.