Participation of Ca2+-acceptor proteins in the mechanisms of the exo-endocytic cycle of synaptic vesicles in the motor nerve endings of the somatic musculature of the earthworm Lumbricus terrestris

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Using fluorescence microscopy, we studied the participation of Ca2+-acceptor proteins in the processes of the exo-endocytotic cycle of neurotransmitter quantal secretion in the neuromuscular junction of the somatic muscle of the earthworm Lumbricus terrestris. Inhibition of calcineurin, calmodulin and Ca2+/calmodulin dependent protein kinases led to an increase in the process of endocytosis. Blocking the phosphorylation of synaptic proteins enhances the process of endocytosis, causes an increase in the size of the total vesicular pool and accelerates the turnover of synaptic vesicles. It can be concluded that calcium modulation of vesicle exo-endocytosis at the synapses of the evolutionarily primary somatic muscles of annelids occurs with the participation of calcineurin, calmodulin and Ca2+/calmodulin-dependent protein kinases.

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Sobre autores

L. Nurullin

Federal Research Center “Kazan Scientific Center of Russian Academy of Sciences”; Kazan State Medical University

Autor responsável pela correspondência
Email: lenizn@yandex.ru

Kazan Institute of Biochemistry and Biophysics

Rússia, Kazan; Kazan

N. Almazov

Kazan State Medical University

Email: lenizn@yandex.ru
Rússia, Kazan

E. Volkov

Kazan State Medical University

Email: euroworm@mail.ru
Rússia, Kazan

Bibliografia

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2. Fig. 1. Fluorescence of neuromuscular synapses of earthworm somatic muscle cells loaded with the lipophilic dye FM2–10. (a) – 0 min, (b) – after 5 min of dye “unloading” at 40 mM K+ in the surrounding solution. Scale bar: 20 µm.

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3. Fig. 2. Effect of DMSO, absence of Ca2+ ions in solution, calcineurin inhibitor – cyclosporin A, Ca2+/calmodulin-dependent protein kinase kinase inhibitor – STO-609, calmodulin antagonist – W-5 hydrochloride on the fluorescence value of motor nerve terminals of the earthworm somatic muscle cell preparation loaded with lipophilic dye FM2–10. a. u. – relative units.

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4. Fig. 3. Effect of calcineurin inhibitor – cyclosporin A, Ca2+/calmodulin-dependent protein kinase kinase inhibitor – STO-609, calmodulin antagonist – W-5 hydrochloride, absence of Ca2+ ions in the surrounding solution (0 mM Ca2+), calcium chelator BAPTA-AM on the “unloading” of motor nerve terminals of the earthworm somatic muscle cell preparation stained with the fluorescent dye FM2–10. In a separate series of experiments, the fading of the FM2–10 dye in a normal solution (4 mM K+) was monitored for 5 min.

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