Colleter Formation on the Leaves of Sweet Potato Microclones (Ipomoea batatas L.) under In Vitro Conditions

R. N. Kirakosyan; Киракосян Р. Н.; Ya. Yu. Golivanov; Голиванов Я. Ю.; V. I. Trukhachev; Трухачев В. И.; S. M. Zaitseva; Зайцева С. М.; M. R. Khaliluev; Халилуев М. Р.; E. N. Baranova; Баранова Е. Н.; E. A. Kalashnikova; Калашникова Е. А.

doi:10.31857/S0015330323600201

Colleter Formation on the Leaves of Sweet Potato Microclones (Ipomoea batatas L.) under In Vitro Conditions

Authors: Kirakosyan R.N.¹, Golivanov Y.Y.², Trukhachev V.I.³, Zaitseva S.M.³, Khaliluev M.R.⁴, Baranova E.N.⁴^,1, Kalashnikova E.A.³
Affiliations:
1. Tsitsin Main Botanical Garden, Russian Academy of Sciences
2. Russian State Agrarian University-Moscow Timiryazev Agricultural Academyж All-Russia Research Institute of Agricultural Biotechnology
3. Russian State Agrarian University-Moscow Timiryazev Agricultural Academy
4. All-Russia Research Institute of Agricultural Biotechnology
Issue: Vol 70, No 6 (2023)
Pages: 612-622
Section: ЭКСПЕРИМЕНТАЛЬНЫЕ СТАТЬИ
URL: https://rjmseer.com/0015-3303/article/view/648161
DOI: https://doi.org/10.31857/S0015330323600201
EDN: https://elibrary.ru/CWCXWX
ID: 648161

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Abstract

Microclones of in vitro vegetable sweet potato (Ipomoea batatas L.) were received and the formation of colors on their leaves was studied. The studies were carried out on three varieties of sweet potato that differ in the color of their pulp and the peel of the tubers as well as in different ripening periods: Purple, Jewel, and Muskatny. The effect of ½ norm of mineral salts MS medium, 1 norm MS and 1½ norm MS on the morphometric parameters of sweet potato microclones was studied. It was established that the use of ½ norm of mineral salts according to MS medium led to the production of rapidly growing axillary and adventitious shoots and the formation of a powerful root system in the basal part of microcuttings. The formation of secretory outgrowths was found for the first time on the leaves of sweet potato microclones when cultured under in vitro conditions. Neoplasms were referred to colleters and their characteristics were given. The dependence of the colleter formation on the microclone leaves on the cycles of culture is shown.

Keywords

Ipomoea batatas, microclones, mineral composition of the culture medium, colleters

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