Scutellaria baicalensis Georgi: Projection of Root Metabolome on Hairy Root Culture

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Abstract

The study characterizes flavonoid and phenylethanoid metabolites of Baikal skullcap (Scutellaria baicalensis). The roots of intact plants and the hairy root cultures were compared. In the culture derived from seeds of wild plants, almost the same set of metabolites as in the whole root was found. Meanwhile, the levels of methylated and glycosylated derivatives of both classes of substances were different. The roots of intact plants accumulated significant amounts of sucrose. Methylated flavones, responsible for plant protection from biotic and abiotic stress-factors, were more abundant in the hairy root cultures. In both specimens, 7‑sulfate 6-ОМе wogonin was identified for the first time. It is concluded that visualization of mass-spectrometric data of a metabolome represents a convenient tool to reveal changes in the metabolome caused by environmental factors or plant damage.

About the authors

Yu. N. Elkin

Elyakov Pacific Institute of Bioorganic Chemistry, Far Eastern Branch, Russian Academy of Sciences

Email: step_ann@mail.ru
690022, Vladivostok, Russia

A. Yu. Manyakhin

Federal Scientific Center of the East Asia Terrestrial Biodiversity, Far Eastern Branch, Russian Academy of Sciences

Email: step_ann@mail.ru
690022, Vladivostok, Russia

A. Yu. Stepanova

Timiryazev Institute of Plant Physiology, Russian Academy of Sciences

Author for correspondence.
Email: step_ann@mail.ru
127276, Moscow, Russia

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Copyright (c) 2023 Ю.Н. Елькин, А.Ю. Маняхин, А.Ю. Степанова