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Effect of Reversion Back to Cys11 on the Structure and Function of S11C Cys-free Nt.BspD6I
- Authors: Artyukh R.I.1, Fatkhullin B.F.2, Antipova V.N.1, Perevyazova T.A.1, Kachalova G.S.1, Yunusova A.K.1
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Affiliations:
- Institute of Theoretical and Experimental Biophysics, Russian Academy of Sciences, 142290, Pushchino, Moscow oblast, Russia
- Institute of Protein Research, Russian Academy of Sciences, 142290, Pushchino, Moscow oblast, Russia
- Issue: Vol 68, No 6 (2023)
- Pages: 859-865
- Section: STRUCTURE OF MACROMOLECULAR COMPOUNDS
- URL: https://rjmseer.com/0023-4761/article/view/673267
- DOI: https://doi.org/10.31857/S0023476123600702
- EDN: https://elibrary.ru/YEJZJU
- ID: 673267
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Abstract
The three-dimensional structure of recombinant nicking endonuclease S11C Cys-free Nt.BspD6I was determined at 1.85 Å resolution. Nickase S11C Cys-free Nt.BspD6I was produced by the reversion back to Cys11 in Cys-free Nt.BspD6I using site-directed mutagenesis. An analysis of the crystal structure of nickase S11C Cys-free Nt.BspD6I demonstrated that the reversion back to Cys11 induces significant conformational changes in the recognition domain of nickase, which are accompanied by changes in its functions, such as a decrease in the affinity to DNA, the loss of the ability to undergo oligomerization, and high activity of restriction endonuclease S11C Cys-free R.BspD6I.
About the authors
R. I. Artyukh
Institute of Theoretical and Experimental Biophysics, Russian Academy of Sciences, 142290, Pushchino, Moscow oblast, Russia
Email: rimmaartyukh@gmail.com
Россия, Пущино
B. F. Fatkhullin
Institute of Protein Research, Russian Academy of Sciences, 142290, Pushchino, Moscow oblast, Russia
Email: rimmaartyukh@gmail.com
Россия, Пущино
V. N. Antipova
Institute of Theoretical and Experimental Biophysics, Russian Academy of Sciences, 142290, Pushchino, Moscow oblast, Russia
Email: rimmaartyukh@gmail.com
Россия, Пущино
T. A. Perevyazova
Institute of Theoretical and Experimental Biophysics, Russian Academy of Sciences, 142290, Pushchino, Moscow oblast, Russia
Email: rimmaartyukh@gmail.com
Россия, Пущино
G. S. Kachalova
Institute of Theoretical and Experimental Biophysics, Russian Academy of Sciences, 142290, Pushchino, Moscow oblast, Russia
Email: rimmaartyukh@gmail.com
Россия, Пущино
A. K. Yunusova
Institute of Theoretical and Experimental Biophysics, Russian Academy of Sciences, 142290, Pushchino, Moscow oblast, Russia
Author for correspondence.
Email: rimmaartyukh@gmail.com
Россия, Пущино
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