Structural Study of the Light-Harvesting Complex LH2 from the Purple Sulfur Bacteria Ectothiorhodospira haloalkaliphila by Cryoelectronic Microscopy

A. D. Burtseva; Бурцева А. Д.; T. N. Baymukhametov; Баймухаметов Т. Н.; I. O. Ilyasov; Илясов И. О.; M. A. Bolshakov; Большаков М. А.; A. A. Moskalenko; Москаленко А. А.; K. M. Boyko; Бойко К. М.; A. A. Ashikhmin; Ашихмин А. А.

doi:10.31857/S0023476123600829

Structural Study of the Light-Harvesting Complex LH2 from the Purple Sulfur Bacteria Ectothiorhodospira haloalkaliphila by Cryoelectronic Microscopy

Authors: Burtseva A.D.¹, Baymukhametov T.N.², Ilyasov I.O.¹, Bolshakov M.A.³, Moskalenko A.A.³, Boyko K.M.¹, Ashikhmin A.A.³
Affiliations:
1. Bach Institute of Biochemistry, Federal Research Center “Fundamentals of Biotechnology” of the Russian Academy of Sciences, 117071, Moscow, Russia
2. National Research Centre “Kurchatov Institute”, 123182, Moscow, Russia
3. Institute of Basic Biological Problems, Russian Academy of Sciences, Federal Research Center “Pushchino Scientific Center for Biological Research” of the Russian Academy of Sciences, Pushchino, Russia
Issue: Vol 68, No 6 (2023)
Pages: 881-887
Section: STRUCTURE OF MACROMOLECULAR COMPOUNDS
URL: https://rjmseer.com/0023-4761/article/view/673272
DOI: https://doi.org/10.31857/S0023476123600829
EDN: https://elibrary.ru/GBGIUU
ID: 673272

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Abstract

The primary processes of photosynthesis for purple photosynthesising bacteria occur in light-harvesting (LH) complexes. The LH2 complex contains polypeptides; bacteriochlorophyll; and, in most cases, carotenoids. There are three known spatial structures of LH2 complexes from purple nonsulfur bacteria; however, high-resolution structures have not been established for purple sulfur bacteria. The results of the structural study of two light-harvesting complexes LH2 from purple sulfur bacteria Ectothiorhodospira haloalkaliphila by cryoelectronic microscopy are reported. Images of carotenoid-containing (LH2+) and carotenoidless (LH2–) variants of the complex, demonstrating a characteristic architecture of the objects of this type, are obtained. A 3D reconstruction of LH2+ is performed with a resolution of 4.5 Å; it coincides with the previously established crystal structure. The presence of particles of different morphology is shown for LH2–.

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