Preparation, Crystallization, and Preliminary X-ray Diffraction Analysis of Recombinant House Dust Mite Allergen Der p 3 from Dermatophagoides pteronyssinus

V. I. Timofeev; Тимофеев В. И.; Yu. A. Abramchik; Абрамчик Ю. А.; N. E. Zhukhlistova; Жухлистова Н. Е.; O. O. Mikheeva; Михеева О. О.; M. B. Shevtsov; Шевцов М. Б.; E. A. Zayats; Заяц Е. А.; D. D. Lykoshin; Лыкошин Д. Д.; M. A. Kostromina; Костромина М. А.; R. S. Esipov; Есипов Р. С.; I. P. Kuranova; Куранова И. П.

doi:10.31857/S0023476122060273

Preparation, Crystallization, and Preliminary X-ray Diffraction Analysis of Recombinant House Dust Mite Allergen Der p 3 from Dermatophagoides pteronyssinus

Авторлар: Timofeev V.I.¹^,2, Abramchik Y.A.¹^,3, Zhukhlistova N.E.¹, Mikheeva O.O.³, Shevtsov M.B.⁴, Zayats E.A.³, Lykoshin D.D.³, Kostromina M.A.³, Esipov R.S.³, Kuranova I.P.¹^,2
Мекемелер:
1. Shubnikov Institute of Crystallography of Federal Scientific Research Centre “Crystallography and Photonics,” Russian Academy of Sciences, 119333, Moscow, Russia
2. National Research Centre “Kurchatov Institute,”, 123098, Moscow, Russia
3. Shemyakin−Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, 117997, Moscow, Russia
4. Moscow Institute of Physics and Technology, 141701, Dolgoprudny, Moscow oblast, Russia
Шығарылым: Том 68, № 1 (2023)
Беттер: 46-50
Бөлім: STRUCTURE OF MACROMOLECULAR COMPOUNDS
URL: https://rjmseer.com/0023-4761/article/view/673539
DOI: https://doi.org/10.31857/S0023476122060273
EDN: https://elibrary.ru/DMUZXL
ID: 673539

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Ашық рұқсат
Рұқсат жабық

Рұқсат берілді
Рұқсат жабық

Тек жазылушылар үшін

Аннотация
Авторлар туралы
Әдебиет тізімі
Қосымша файлдар
Статистика

Аннотация

The high-producing strain С3029/pGro7/pERDerp3 for the house dust mite allergen Der p 3 from Dermatophagoides pteronyssinus, expressing the recombinant protein in Escherichia coli in the soluble form, was constructed. A procedure was developed for the purification of the recombinant allergen. Crystals of the recombinant protein Der p 3 suitable for X-ray diffraction analysis were grown by the vapor-diffusion method. The X-ray diffraction data set was collected to 2.25 Å resolution at the European Synchrotron Radiation Facility (ESRF, France, ID23-1 beamline) at 100 K. The crystals belong to sp. gr. С121 and contain two enzyme molecules per asymmetric unit.

Негізгі сөздер

STRUCTURE OF MACROMOLECULAR COMPOUNDS

Авторлар туралы

V. Timofeev

Shubnikov Institute of Crystallography of Federal Scientific Research Centre “Crystallography and Photonics,” Russian Academy of Sciences, 119333, Moscow, Russia; National Research Centre “Kurchatov Institute,”, 123098, Moscow, Russia

Email: inna@crys.ras.ru
Россия, Москва; Россия, Москва

Yu. Abramchik

Shubnikov Institute of Crystallography of Federal Scientific Research Centre “Crystallography and Photonics,” Russian Academy of Sciences, 119333, Moscow, Russia; Shemyakin−Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, 117997, Moscow, Russia

Email: inna@crys.ras.ru
Россия, Москва; Россия, Москва

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