DEVELOPMENT OF X-RAY METHODS FOR STUDYING PROTEIN PLANAR SYSTEMS ON A LIQUID SURFACE USING SYNCHROTRON RADIATION

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Abstract

The structure of Langmuir lysozyme films on a liquid surface, formed from crystallization solutions with addition of metal chlorides as a precipitant, have been investigated. The thicknesses and densities of the films were determined using the X-ray reflectivity technique, and the concentration distribution profiles of the sulfur atoms present in protein molecules, as well as precipitant ions in the subphase surface region, have been obtained by the X-ray standing waves technique. Based on the experimental results, the dependence of the film structure on the precipitant used, as well as some specific features of application of X-ray reflectivity and X-ray standing waves techniques in the study of Langmuir films of globular proteins on a liquid surface, are analyzed.

About the authors

M. S. Folomeshkin

National Research Centre “Kurchatov Institute”, 123182, Moscow, Russia; Shubnikov Institute of Crystallography, Federal Scientific Research Centre “Crystallography and Photonics,” Russian Academy of Sciences, 119333, Moscow, Russia

Email: folmaxim@gmail.com
Россия, Москва; Россия, Москва

A. E. Blagov

National Research Centre “Kurchatov Institute”, 123182, Moscow, Russia; Shubnikov Institute of Crystallography, Federal Scientific Research Centre “Crystallography and Photonics,” Russian Academy of Sciences, 119333, Moscow, Russia

Email: folmaxim@gmail.com
Россия, Москва; Россия, Москва

A. S. Boikova

National Research Centre “Kurchatov Institute,” Moscow, 123182 Russia

Email: folmaxim@gmail.com
Россия, Москва

Yu. A. Volkovsky

National Research Centre “Kurchatov Institute”, 123182, Moscow, Russia; Shubnikov Institute of Crystallography, Federal Scientific Research Centre “Crystallography and Photonics,” Russian Academy of Sciences, 119333, Moscow, Russia

Email: folmaxim@gmail.com
Россия, Москва; Россия, Москва

Yu. A. Dyakova

Shubnikov Institute of Crystallography, Federal Scientific Research Centre “Crystallography and Photonics,” Russian Academy of Sciences, Moscow, 119333 Russia; National Research Centre “Kurchatov Institute,” Moscow, 123182 Russia

Email: folmaxim@gmail.com
Россия, Москва; Россия, Москва

K. B. Ilina

Shubnikov Institute of Crystallography, Federal Scientific Research Centre “Crystallography and Photonics,” Russian Academy of Sciences, Moscow, 119333 Russia; National Research Centre “Kurchatov Institute,” Moscow, 123182 Russia

Email: folmaxim@gmail.com
Россия, Москва; Россия, Москва

M. A. Marchenkova

Shubnikov Institute of Crystallography, Federal Scientific Research Centre “Crystallography and Photonics,” Russian Academy of Sciences, Moscow, 119333 Russia; National Research Centre “Kurchatov Institute,” Moscow, 123182 Russia

Email: folmaxim@gmail.com
Россия, Москва; Россия, Москва

Yu. V. Pisarevsky

National Research Centre “Kurchatov Institute”, 123182, Moscow, Russia; Shubnikov Institute of Crystallography, Federal Scientific Research Centre “Crystallography and Photonics,” Russian Academy of Sciences, 119333, Moscow, Russia

Email: folmaxim@gmail.com
Россия, Москва; Россия, Москва

P. A. Prosekov

National Research Centre “Kurchatov Institute”, 123182, Moscow, Russia; Shubnikov Institute of Crystallography, Federal Scientific Research Centre “Crystallography and Photonics,” Russian Academy of Sciences, 119333, Moscow, Russia

Email: folmaxim@gmail.com
Россия, Москва; Россия, Москва

A. V. Rogachev

National Research Centre “Kurchatov Institute,” Moscow, 123182 Russia

Email: folmaxim@gmail.com
Россия, Москва

A. Yu. Seregin

National Research Centre “Kurchatov Institute”, 123182, Moscow, Russia; Shubnikov Institute of Crystallography, Federal Scientific Research Centre “Crystallography and Photonics,” Russian Academy of Sciences, 119333, Moscow, Russia

Email: folmaxim@gmail.com
Россия, Москва; Россия, Москва

M. V. Kovalchuk

National Research Centre “Kurchatov Institute”, 123182, Moscow, Russia; Shubnikov Institute of Crystallography, Federal Scientific Research Centre “Crystallography and Photonics,” Russian Academy of Sciences, 119333, Moscow, Russia; St. Petersburg State University, 199034, St.Petersburg, Russia

Author for correspondence.
Email: folmaxim@gmail.com
Россия, Москва; Россия, Москва; Россия, Санкт-Петербург

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