Artificial modified nucleotides for the electrochemical detection of nucleic acid amplification products

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About the authors

Е. V. Suprun

Moscow State University; Orekhovich Research Institute of Biomedical Chemistry

Author for correspondence.
Email: lenasuprun@mail.ru

химический факультет

Russian Federation, 119991, Moscow; 119121, Moscow

S. A. Khmeleva

Orekhovich Research Institute of Biomedical Chemistry

Email: lenasuprun@mail.ru
Russian Federation, 119121, Moscow

K. G. Ptitsyn

Orekhovich Research Institute of Biomedical Chemistry

Email: lenasuprun@mail.ru
Russian Federation, 119121, Moscow

L. K. Kurbatov

Orekhovich Research Institute of Biomedical Chemistry

Email: lenasuprun@mail.ru
Russian Federation, 119121, Moscow

S. P. Radko

Orekhovich Research Institute of Biomedical Chemistry

Email: lenasuprun@mail.ru
Russian Federation, 119121, Moscow

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Supplementary files

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2. 1. Modification of nucleotides by electroactive groups. Using the example of a DNA nucleotide.

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3. 2. The diversity of electroactive groups and linkers in synthetic modified nucleotides: ferrocene [74-77, 103-105], anthraquinone [83, 91], 4-hydroxy-3-methoxyphenyl and 2,3-dihydrobenzofuran-5-yl [84], phenothiazine [85], tyrosine and tryptophan residues [88, 89], 3-aminophenyl and 3-nitrophenyl [87, 106], benzofurazane [98]. L – linker, P – electroactive functional group.

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4. 3. The main fields of application of artificial electroactive nucleotides.

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5. 4. Ferrocene and its derivatives as electroactive nucleotide labels: ferrocene carboxamide (1), unsubstituted ferrocene (2), octamethylferrocene (3), ferrocenvinyl (4), 5-(3-ferrocene carboxamidopropenyl-1) (5) and 5-(3-ferrocenacetamidopropenyl-1) (6) linked by a linker to the carbon atom C7 or C5 of the purine or pyrimidine nitrogenous base of the nucleotide (DNA or RNA), respectively [74-78, 103-109, 120]. For modified DBPs, the oxidation peak potentials increase from (1) to (3) [75], from (4) to (5) [106], and from (6) to (5) [108].

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6. 5. Combining the isothermal amplification reaction with the introduction of modified nucleotides and voltammetric recording of the yield is a way to determine viral or bacterial infection in situ.

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