Hybrid Organomineral Carriers for Therapeutic Proteins

Capa

Citar

Texto integral

Acesso aberto Acesso aberto
Acesso é fechado Acesso está concedido
Acesso é fechado Acesso é pago ou somente para assinantes

Resumo

Drug delivery systems are designed to provide the necessary concentration and prolonged effect of the active substance in the body. Orally administered protein preparations require protection from proteolysis in the gastrointestinal tract. Biocompatible hydrophilic polysaccharides in the matrix are especially promising, since they do not irritate the intestines and are gradually cleaved by specific glycosidases, releasing the therapeutic agent. The introduction of an insoluble porous mineral matrix into composition of carrier makes it possible to increase the concentration of therapeutic in matrix without a significant increase in the volume of drug tablet form. In this work, a new original organic mineral carrier based on heat-treated crushed clinoptiolite zeolite in combination with natural polysaccharides from red seaweeds – agar-agar, agarose, and carrageenan was created. Granular and finely dispersed clinoptiolites in the matrix are loaded with a promising therapeutic agent, Bacillus pumilus ribonuclease (binase), which exhibits selective cytotoxicity to tumor cells. It has been established that both granular and finely dispersed zeolites in combination with polysaccharides retain protein better than pure zeolites and provide a gradual complete release of enzyme in 18 hours. At the same time, the enzyme retains its catalytic activity and induces apoptosis up to 23.8% cells of the human duodenal adenocarcinoma HuTu80. The data obtained substantiate the prospects of dosage forms based on the used organomineral carriers designing.

Sobre autores

P. Zelenikhin

Kazan (Volga Region) Federal University

Autor responsável pela correspondência
Email: pasha_mic@mail.ru
Russia, 420008, Kazan, ul. Kremlevskaya 18

A. Galeeva

Kazan (Volga Region) Federal University; Federal Center for Toxicological, Radiation and Biological Safety

Email: pasha_mic@mail.ru
Russia, 420008, Kazan, ul. Kremlevskaya 18; Russia, 420075, Kazan, Nauchny gorodok-2

R. Islamova

Kazan (Volga Region) Federal University

Email: pasha_mic@mail.ru
Russia, 420008, Kazan, ul. Kremlevskaya 18

O. Lopatin

Kazan (Volga Region) Federal University

Email: pasha_mic@mail.ru
Russia, 420008, Kazan, ul. Kremlevskaya 18

R. Yarullin

JSC “Tatneftekhiminvest-Holding”

Email: pasha_mic@mail.ru
Russia, 420061, Kazan, ul. Nikolay Ershov 29A

O. Ilinskaya

Kazan (Volga Region) Federal University

Email: pasha_mic@mail.ru
Russia, 420008, Kazan, ul. Kremlevskaya 18

Bibliografia

  1. Бондарев А.В., Жилякова Е.Т. // Фармация и фармакология. 2019. Т. 7. С. 4–12. https://doi.org/10.19163/2307-9266-2019-7-1-4-12
  2. Бондарев А.В., Жилякова Е.Т., Демина Н.Б., Размахнин К.К. // Разработка и регистрация лекарственных средств. 2021. Т. 10. С. 65–71. https://doi.org/10.33380/2305-2066-2021-10-4-65-71
  3. Chirani N., Yahia L’H., Gritsch L., Motta F.L., Chirani S., Fare S. // J. Biomed. Sci. 2015. V. 4. P. 1–23. https://doi.org/10.4172/2254-609X.100013
  4. Kouser R., Vashist A., Zafaryab M., Rizvi M.A., Ahmad S. // ACS Appl. Bio Mater. 2018. V. 1. P. 1810–1822. https://doi.org/10.1021/acsabm.8b00260
  5. Liu X., Steiger C., Lin S., Parada G.A., Liu J., Chan H.F., Yuk H., Phan N.V., Collins J., Tamang S., Traverso G., Zhao X. // Nat. Commun. 2019. V. 1. P. 493. https://doi.org/10.1038/s41467-019-08355-2
  6. Norouzi M., Nazari B., Miller D.W. // Drug Discov. Today. 2016. V. 21. P. 1835–1849. https://doi.org/10.1016/j.drudis.2016.07.006
  7. Gao W., Zhang Y., Zhang Q., Zhang L. // Ann. Biomed. Eng. 2016. V. 44. P. 2049–2061. https://doi.org/10.1007/s10439-016-1583-9
  8. Ivanova I.I., Knyazeva E.E. // Chem. Soc. Rev. 2013. V. 42. P. 3671–3688. https://doi.org/10.1039/c2cs35341e
  9. Attanoos R.L., Churg A., Galateau-Salle F., Gibbs A.R., Roggli V.L. // Arch. Path. Lab. 2018. V. 142. P. 753–760. https://doi.org/10.5858/arpa.2017-0365-RA
  10. Khojaewa V., Lopatin O., Zelenikhin P., Ilinskaya O. // Front. Pharmacol. 2019. V. 10. P. 442. https://doi.org/10.3389/fphar.2019.00442
  11. Mastinu A., Kumar A., Maccarinelli G., Bonini S.A., Premoli M., Aria F., Gianoncelli A., Memo M. // Molecules. 2019. V. 24. P. 1517. https://doi.org/10.3390/molecules24081517
  12. Kraljević Pavelić S., Simović Medica J., Gumbarević D., Filošević A., Pržulj N., Pavelić K. // Front. Pharmacol. 2018. V. 9. P. 1350. https://doi.org/10.3389/fphar.2018.01350
  13. Исламова Р.Р., Яковлева Г.Ю., Ильинская О.Н., Лопатин О.Н. // Матер. международ. науч. конфер. “Проблемы геологии и расширение минерально-сырьевой базы стран Евразии”, Алматы. 2019. С. 317–322. ISBN 978-601-332-416-6.
  14. Исламова Р.Р., Яковлева Г.И., Тюрин А.Н., Ильинская О.Н., Лопатин О.Н. // Записки российского минералогического общества. 2022. Т. 151. С. 105–113.
  15. Makarov A.A., Kolchinsky A., Ilinskaya O.N. // BioEssays. 2008. V. 30. P. 781–790. https://doi.org/10.1002/bies.20789
  16. Garipov A.R., Nesmelov A.A., Cabrera-Fuentes H.A., Ilinskaya O.N. // Toxicon. 2014. V. 92. P. 54–59. https://doi.org/10.1016/j.toxicon.2014.09.014
  17. Mohamed I.S.E., Sen’kova A.V., Nadyrova A.I., Savin I.A., Markov A.V., Mitkevich V.A., Makarov A.A., Ilinskaya O.N., Mironova N.L., Zenkova M.A. // Biomolecules. 2020. V. 10. P. 1509. https://doi.org/10.3390/biom10111
  18. Bogdanova L.R., Zelenikhin P.V., Makarova A.O., Zueva O.S., Salnikov V.V., Zuev Y.F., Ilinskaya O.N. // Polymers (Basel). 2022. V. 14. P. 2461. https://doi.org/10.3390/polym14122461
  19. Khodzhaeva V., Makeeva A., Ulyanova V., Zelenikhin P., Evtugyn V., Hardt M., Rozhina E., Lvov Y., Fakhrullin R., Ilinskaya O. // Front. Pharmacol. 2017. V. 8. P. 631. https://doi.org/10.3389/fphar.2017.00631
  20. Ilinskaya O., Ulyanova V., Lisevich I., Dudkina E., Zakharchenko N., Kusova A., Faizullin D., Zuev Y. // Biomed. Res. Int. 2018. P. 4837623. https://doi.org/10.1155/2018/4837623
  21. Cui S., Arosio D., Doherty K.M., Brosh R.M., Jr., Falaschi A., Vindigni A. // Nucleic Acids Res. 2004. V. 32. P. 2158–2170. https://doi.org/10.1093/nar/gkh540
  22. Wasilewska M., Adamczyk Z., Jachimska B. // Langmuir. 2009. V. 25. P. 3698–3704. https://doi.org/10.1021/la803662a
  23. Dudkina E., Ulyanova V., Shah Mahmud R., Khodzhaeva V., Dao L., Vershinina V., Kolpakov A., Ilinskaya O. // FEBS Open Biol. 2016. V. 6. P. 24‒32. https://doi.org/10.1002/feb4.12023
  24. Foglar L., Sipos L., Bolf N. // World J. Microbiol. Biotechnol. 2007. V. 23. P. 1595–1603. https://doi.org/10.1007/s11274-007-9405-8
  25. Колпаков А.И., Ильинская О.Н. // Клиническая и лабораторная диагностика. 1999. № 5. С. 14‒16.
  26. Пирузян А.В., Боковикова Т.Н., Найденов Ю.В. // Электронный научно-технический журнал “Физико-химический анализ свойств многокомпонентных систем”. 2008. № 6. https://fh.kubstu.ru/fams/issues/issue06/st0630.pdf

Arquivos suplementares

Arquivos suplementares
Ação
1. JATS XML
Baixar
2.

Baixar (1MB)
Metadados
3.

Baixar (218KB)
Metadados
4.

Baixar (347KB)
Metadados
5.

Baixar (301KB)
Metadados

Declaração de direitos autorais © П.В. Зеленихин, А.Г. Галеева, Р.Р. Исламова, О.Н. Лопатин, Р.С. Яруллин, О.Н. Ильинская, 2023