Population growth of microalga Tisochrysis lutea (Haptophyta) and the content of carotenoids and neutral lipids at the different illuminations in a panel bioreactor

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Abstract

The effect of illumination (10, 30, 50, and 70 μmol/m2/s) on microalga Tisochrysis lutea (Haptophyta) in a 1.8 L panel bioreactor was studied using a 5 Lux–LED flat panel (Infors HT, Switzerland) for 14 days. It was found that the most intensive growth of microalga occurred at 30 and 50 μmol/m²/s. At 10 μmol/m²/s, population growth was completely suppressed. Regardless of the light intensity, cells larger than 4 μm predominated in the algae population after seven days. The carotenoid content at illuminations of 10 and 70 μmol/m²/s was highest on the seventh day. The chlorophyll a to carotenoid ratio decreased by the end of the experiment in all conditions. The highest amount of neutral lipids in cells was noted at 10 μmol/m²/s.

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About the authors

Zh. V. Маrkina

A.V. Zhirmunsky National Scientific Center of Marine Biology, Far Eastern Branch, Russian Academy of Sciences

Author for correspondence.
Email: zhannav@mail.ru
ORCID iD: 0000-0001-7135-1375
Russian Federation, Vladivostok 690041

A. A. Zinov

A.V. Zhirmunsky National Scientific Center of Marine Biology, Far Eastern Branch, Russian Academy of Sciences

Email: zhannav@mail.ru
ORCID iD: 0000-0003-4705-5941
Russian Federation, Vladivostok 690041

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2. Fig. 1. Population growth of Tisochrysis lutea under different illumination. Different Latin letters indicate statistically significant differences between the average values ​​for each day of the experiment at p <0.05.

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3. Fig. 2. Size structure of the Tisochrysis lutea population under different illumination conditions: (a) – 10 μmol/m2/s, (b) – 30 μmol/m2/s, (c) – 50 μmol/m2/s, (d) – 70 μmol/m2/s.

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4. Fig. 3. Total carotenoid content in Tisochrysis lutea under different illumination. Different Latin letters indicate statistically significant differences between the average values ​​for each day of the experiment at p <0.05.

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5. Fig. 4. Fluorescence of Tisochrysis lutea cells stained with Nile Red, an indicator of neutral lipid content. Different Latin letters indicate statistically significant differences between the mean values ​​for each day of the experiment at p <0.05.

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