Lipid Membrane Electroporation Cannot Be Described by the Constant Line Tension Model of the Pore Edge

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Abstract

We have studied the process of electroporation of bilayer lipid membranes (BLMs) from dioleoylphosphatidylcholine (DOPC). We obtained experimental data on the average lifetime of the membrane as a function of applied voltage in the range of 200–375 mV. The analysis of the data obtained showed that the dependence is nonmonotonic and cannot be described in terms of the classical theory of electroporation. These results are consistent with modern models of the process of through conductive pores formation in a membrane. The above models imply a complex pore energy profile and its dependence on membrane tension and external electric field. Thus, we have shown that the classical theory of electroporation does not satisfy the experimentally observed dependencies of the average membrane lifetime on the applied potential difference and requires further refinement.

About the authors

P. K. Gifer

Frumkin Institute of Physical Chemistry and Electrochemistry, Russian Academy of Sciences; Moscow Institute of Physics and Technology

Author for correspondence.
Email: gifer.pk@phystech.edu
Russia, 119071, Moscow; Russia, 141700, Moscow oblast, Dolgoprudniy

O. V. Batishchev

Frumkin Institute of Physical Chemistry and Electrochemistry, Russian Academy of Sciences

Email: gifer.pk@phystech.edu
Russia, 119071, Moscow

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