Mixed Cationic Liposomes Based on L-Amino Acids As Efficient Delivery Systems of Therapeutic Molecules into Cells

Мұқаба

Дәйексөз келтіру

Толық мәтін

Ашық рұқсат Ашық рұқсат
Рұқсат жабық Рұқсат берілді
Рұқсат жабық Тек жазылушылар үшін

Аннотация

This work aimed to produce mixed liposomes based on natural amino acids as vehicles for delivery of anticancer drugs and nucleic acids. Liposomes were formed from cationic lipids based on L-alanine and L-serine, a kerase-forming lipid based on L-ornithine, and phospholipids phosphatidylcholine (PC) or 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine (DOPE). For the developed agents, particle size, zeta potential, and stability were determined, and the biological activity was studied on the MCF-7 and HEK 293 cell lines. Liposomes based on L-serine demonstrated the ability to accumulate in the endoplasmic reticulum of cells within 1 h, and their transfection activity significantly exceeded that of the commercial drug Lipofectamine-2000. At the same time, the proposed system had a slight toxic effect (IC50, 0.475 mg/mL and the safe working concentration, 0.24 mg/mL).

Авторлар туралы

Z. Denieva

Frumkin Institute of physical chemistry and electrochemistry, Russian Academy Of Sciences

Хат алмасуға жауапты Автор.
Email: zaret03@mail.ru
Russia, 119071, Moscow

O. Koloskova

NRC Institute of Immunology FMBA of Russia

Email: zaret03@mail.ru
Russia, 115522, Moscow

A. Gileva

Shemyakin–Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences

Email: zaret03@mail.ru
Russia, 117997, Moscow

U. Budanova

MIREA – Russian Technology University (Lomonosov Institute of Fine Chemical Technology)

Email: zaret03@mail.ru
Russia, 119571, Moscow

Yu. Sebyakin

MIREA – Russian Technology University (Lomonosov Institute of Fine Chemical Technology)

Email: zaret03@mail.ru
Russia, 119571, Moscow

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