Reversible Increase in Resistance of A-431 Carcinoma Cells to TRAIL-Induced Apoptosis in Confluent Cultures Corresponds to a Decrease in Expression of DR4 and DR5 Receptors

R. S. Fadeev; Фадеев Р. С.; N. V. Dolgikh; Долгих Н. В.; A. V. Chekanov; Чеканов А. В.; A. S. Senotov; Сенотов А. С.; K. S. Krasnov; Краснов К. С.; M. I. Kobyakova; Кобякова М. И.; Ya. V. Lomovskaya; Ломовская Я. В.; I. S. Fadeevа; Фадеева И. С.; V. S. Akatov; Акатов В. С.

doi:10.31857/S0233475523020020

Reversible Increase in Resistance of A-431 Carcinoma Cells to TRAIL-Induced Apoptosis in Confluent Cultures Corresponds to a Decrease in Expression of DR4 and DR5 Receptors

Authors: Fadeev R.S.¹^,2, Dolgikh N.V.¹, Chekanov A.V.¹, Senotov A.S.¹, Krasnov K.S.¹^,2, Kobyakova M.I.¹, Lomovskaya Y.V.¹, Fadeevа I.S.¹^,2, Akatov V.S.¹^,2
Affiliations:
1. Institute for Theoretical and Experimental Biophysics, Russian Academy of Sciences
2. Puschino State Natural Science Institute
Issue: Vol 40, No 2 (2023)
Pages: 105-111
Section: Articles
URL: https://rjmseer.com/0233-4755/article/view/667372
DOI: https://doi.org/10.31857/S0233475523020020
EDN: https://elibrary.ru/LALOSK
ID: 667372

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Abstract

TRAIL (TNF alpha Related Apoptosis Inducing Ligand) cytokine is of great interest for the development of targeted antitumor drugs. We have previously found a reversible increase in tumour cell resistance to TRAIL-induced apoptosis in confluent cultures. In this work we show that increase in resistance of A-431 cells to TRAIL-induced death in confluent culture is associated with reduced expression of pro-apoptotic receptors DR4 and DR5 with absence of anti-apoptotic receptors DcR1 and DcR2 on cell surface. Decreased representation of DR4 and DR5 receptors on the cell surface is accompanied by a lack of activation of the pro-apoptotic protein Bid, effector caspase 3 under the action of recombinant protein izTRAIL, which leads to an increase in TRAIL resistance. Our results indicate that reversible increase in resistance of human carcinoma A-431 cells to TRAIL-induced apoptosis in confluent cultures is caused by decrease in expression of DR4 and DR5 receptors on cell surface.

Keywords

human carcinoma cells, TRAIL-induced apoptosis, confluent culture, TRAIL resistance, TRAIL receptors

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