Production of Reactive Oxygen Species by Genetically Encoded Photosensitizers 4D5scFv-miniSOG and DARPin-miniSOG in Living Cells

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Abstract

Currently, experimental oncology is developing approaches based on the development of targeted photosensitizers that can be delivered specifically to a tumor of a certain molecular profile. This work is devoted to the study of the generation of reactive oxygen species by photosensitizers of protein nature 4D5scFv-miniSOG and DARPin-miniSOG, specific to the oncomarker HER2. It was found that despite the presence of the same phototoxic module in their composition, these photosensitizers are characterized by different levels of ROS production, which may be explained by the different rate of receptor-mediated internalization of 4D5scFv-miniSOG and DARPin-miniSOG.

About the authors

G. M. Proshkina

Shemyakin–Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences

Author for correspondence.
Email: gmb@ibch.ru
Russia, 117997, Moscow

E. I. Shramova

Shemyakin–Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences

Email: gmb@ibch.ru
Russia, 117997, Moscow

S. M. Deyev

Shemyakin–Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences; Sechenov First Moscow State Medical University of the Ministry of Health of the Russian Federation (Sechenov University)

Email: gmb@ibch.ru
Russia, 117997, Moscow; Russia, 119991, Moscow

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