Organization of the Reserve Pool of Synaptic Vesicles in Nerve Terminals Lacking Protein Liquid Phase Components

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Abstract

Protein endophilin A regulates the synaptic vesicle cycle during exo- and endocytosis, and it is present in the reserve pool of synaptic vesicles (SVs), where its function is unknown. In vitro experiments suggest that endophilin via its SH3 domain interactions incorporates several components into the protein liquid phase that organizes SVs in the reserve pool. We investigated the effect of deletion of the genes encoding endophilin and one of its binding partners, dynamin, on the organization of SVs in living synapses formed by cortical neurons in culture. Experiments showed that deletion of endophilin genes does not change the density of SVs in the reserve pool. At the same time, deletion of the major dynamins 1 and 3 leads to a significant increase in the vesicle density. These results suggest that other SH3-domain-containing proteins, which are components of the protein liquid phase, complement the function of endophilin in the SV reserve pool.

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About the authors

N. V. Nifantova

Institute of Translational Biomedicine, St. Petersburg State University

Author for correspondence.
Email: oleg.shupliakov@ki.se
Russian Federation, St. Petersburg, 199034

A. G. Shishkov

Institute of Translational Biomedicine, St. Petersburg State University

Email: oleg.shupliakov@ki.se
Russian Federation, St. Petersburg, 199034

O. M. Korenkova

Institute of Translational Biomedicine, St. Petersburg State University

Email: oleg.shupliakov@ki.se
Russian Federation, St. Petersburg, 199034

E. Sopova

Institute of Translational Biomedicine, St. Petersburg State University; Karolinska Institutet

Email: oleg.shupliakov@ki.se
Russian Federation, St. Petersburg, 199034; Stockholm, 17177, Sweden

L. Brodin

Karolinska Institutet

Email: oleg.shupliakov@ki.se
Sweden, Stockholm, 17177

O. Shupliakov

Institute of Translational Biomedicine, St. Petersburg State University; Karolinska Institutet

Email: oleg.shupliakov@ki.se
Russian Federation, St. Petersburg, 199034; Stockholm, 17177, Sweden

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2. Fig. 1. Effects of deletion of endophilin and dynamin genes on the organization of the CB reserve pool in asymmetric synapses in cortical neuron culture.

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