Production of Soluble Human Gamma Interferon in the  Escherichia coli  Expression System with a Decrease in Cultivation Temperature

E. A. Volosnikova; Волосникова Е. А.; T. I. Esina; Есина Т. И.; D. N. Shcherbakov; Щербаков Д. Н.; N. V. Volkova; Волкова Н. В.; Ya. S. Gogina; Гогина Я. С.; T. A. Tereshchenko; Терещенко Т. А.; E. D. Danilenko; Даниленко Е. Д.

doi:10.31857/S0555109923020174

Production of Soluble Human Gamma Interferon in the Escherichia coli Expression System with a Decrease in Cultivation Temperature

作者: Volosnikova E.A.¹, Esina T.I.¹, Shcherbakov D.N.¹, Volkova N.V.¹, Gogina Y.S.¹, Tereshchenko T.A.¹, Danilenko E.D.¹
隶属关系:
1. State Research Center of Virology and Biotechnology “Vector”
期: 卷 59, 编号 2 (2023)
页面: 167-173
栏目: Articles
URL: https://rjmseer.com/0555-1099/article/view/674632
DOI: https://doi.org/10.31857/S0555109923020174
EDN: https://elibrary.ru/LVLPXU
ID: 674632

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详细

A recombinant strain producing human gamma interferon (IFN-γ) E. coli BL 21/pET-IFN-γ was constructed, providing a high level of its expression. A method has been developed for obtaining a soluble form of recombinant IFN-γ, consisting of the processes of producing a biomass of a producer strain containing a target protein in an amount of 32–37% of the total content of cellular proteins, protein isolation and purification. The purification process included the stages of disintegration, clarification of the cell lysate, chromatographic purification and dialysis. The developed method makes it possible to obtain from 1 g of wet biomass up to 5 mg of the drug with a purity of at least 95% and high specific (antiviral) activity.

关键词

human recombinant gamma interferon, producer strain, cultivation, chromatography, specific activity

参考

Pandey R., Prabhu A.A., Dasu V.V. // Separation Science and Technology. 2018. V. 53. № 3. P. 487–495. https://doi.org/10.1080/01496395.2017.1395463
Razaghi A., Owens L., Heimann K. // J. Biotechnol. 2016. V. 240. P. 48–60. https://doi.org/10.1016/j.jbiotec.2016.10.022
Khalilzadeh R., Shojaosadati S.A., Bahrami A., Maghsoudi N. // Biotechnology Letters. 2003. V. 25. № 23. P. 1989–1992. https://doi.org/10.1023/b:bile.0000004390.98648.25
Rosano G.L., Ceccarelli E.A. // Front. Microbiol. 2014. V. 5. P. 172. https://doi.org/10.3389/fmicb.2014.00172
Malhotra A. // Methods in Enzymol. 2009. V. 463. P. 239–258. https://doi.org/10.1016/S0076-6879(09)63016-0
Leibly D.J., Nguyen T.N., Kao L.T., Hewitt S.N., Barrett L.K., Van Voorhis W.C. // PlOS ONE. 2012. V. 7. №. 12. P. e52482. https://doi.org/10.1371/journal.pone.0052482
Tileva M., Krachmarova E., Ivanov I., Maskos K., Nacheva G. // Protein Expr. Purif. 2016. V. 117. P. 26–34. https://doi.org/10.1016/j.pep.2015.09.022
Государственная фармакопея РФ XIV изд. Т.2. ОФС.1.7.2.0002.15 Биологические методы испытания препаратов интерферона с использованием культур клеток. С. 2740–2749. https://femb.ru/record/pharmacopea14
Хайруллин Р.Ф. Экспрессия рекомбинантных белков в E. coli: учебн. пособие. / Ред. Р.Ф. Хайруллин, Р.Г. Киямова, А.А. Ризванов. Казань: Изд-во Казанского ун-та, 2018. 142 с.
Закабунин А.И., Барановская Г.А., Пустошилова Н.М., Майстренко В.Ф., Гаврюченкова Л.П., Громова О.А. Способ получения рекомбинантного интерферона – гамма человека. Патент РФ. 1999. № 2132386.