The Effect of Prolonged Emotional and Painful Stress on the Expression of Proinflammatory Cytokine Genes in Rats with High and Low Excitability of the Nervous System

I. G. Shalaginova; Шалагинова И. Г.; O. P. Tuchina; Тучина О. П.; A. V. Turkin; Туркин А. В.; A. E. Vylegzhanina; Вылегжанина А. Э.; A. N. Nagumanova; Нагуманова А. Н.; T. G. Zachepilo; Зачепило Т. Г.; M. B. Pavlova; Павлова М. Б.; N. A. Dyuzhikova; Дюжикова Н. А.

doi:10.31857/S0869813923040088

The Effect of Prolonged Emotional and Painful Stress on the Expression of Proinflammatory Cytokine Genes in Rats with High and Low Excitability of the Nervous System

Authors: Shalaginova I.G.¹, Tuchina O.P.¹, Turkin A.V.¹, Vylegzhanina A.E.¹, Nagumanova A.N.¹, Zachepilo T.G.², Pavlova M.B.², Dyuzhikova N.A.²
Affiliations:
1. Immanuel Kant Baltic Federal University
2. Pavlov Institute of Physiology, Russian Academy of Sciences
Issue: Vol 109, No 4 (2023)
Pages: 545-558
Section: EXPERIMENTAL ARTICLES
URL: https://rjmseer.com/0869-8139/article/view/651583
DOI: https://doi.org/10.31857/S0869813923040088
EDN: https://elibrary.ru/VJNHJJ
ID: 651583

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Abstract

Stress plays an important role in the pathogenesis of anxiety and depressive disorders. Neuroinflammation is considered as one of the mechanisms by which stress alters the molecular and cellular plasticity in the nervous tissue, which leads to a violation of the functions of the central nervous system. The contribution of genetically determined features of the nervous system to the development of post-stress neuroinflammation has not been sufficiently studied. In this study, the dynamics of poststress changes in the mRNA levels of the il1ß and tnf genes of proinflammatory cytokines interleukin-1-beta (IL-1ß) and tumor necrosis factor (TNF) in the blood and in the brain in two rat strains with high and low excitability thresholds of the nervous system (HT and LT) was evaluated. Changes in IL-1ß and TNF mRNA levels were assessed by real-time PCR 24 h, 7, 24 and 60 days after prolonged emotional and painful stress in the blood and three brain structures involved in the development of post-stress pathology (prefrontal cortex, hippocampus, amygdala). In highly excitable rats of the LT strain, the level of IL-1ß mRNA in the hippocampus and amygdala increased compared to the control 24 days after the end of stress, in low-excitable animals of the HT strain, an increase in the level of IL-1ß mRNA was detected only in the hippocampus at the same time. The TNF mRNA level did not change in any of the strains at any of the time points after stress. Genetically determined excitability of the nervous system is a promising marker of individual vulnerability to stress, manifested in post-stress disorders associated with the characteristics of the formation and dynamics of neuroinflammation.

Keywords

neuroinflammation, proinflammatory cytokines, IL-1ß, tnf, stress, excitability of the nervous system, post-stress disorders, rats

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