Effect of some metabiotics from strains of the genus Enterococcus on the growth of pathogenic bacteria that cause pneumonia

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Ашық рұқсат Ашық рұқсат
Рұқсат жабық Рұқсат берілді
Рұқсат жабық Рұқсат ақылы немесе тек жазылушылар үшін

Аннотация

It is shown that the highest antimicrobial activity (100%) against strains of Kl. pneumonia and St. pneumonia of strains causing pneumonia occurs when using protein-like fractions (AMP) obtained from CL after cultivation of LAB of strains of the genus Enterococcus isolated from fermented milk of goats and donkeys, and the lowest activity of AMP from CL of strains isolated from fermented milk of cows (20–50%). It was shown that the isolated polysaccharides of strains of the genus Enterococcus, which have antimicrobial activity, consist of glucose and galactose molecules. The difference in the effectiveness of the influence of polysaccharides depends on the source of the isolated pathogens, depends on the concentration of the polysaccharide, the genus and species of the strain causing pneumonia. It is concluded that some metabiotics of the Enterococcus genus are promising for use as bioinhibitors of the growth of pathogenic bacteria.

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Рұқсат жабық

Авторлар туралы

А. Israyelyan

Artsakh Scientific Center State Non-Commercial Organization

Хат алмасуға жауапты Автор.
Email: arevik_israelyan@mail.ru
Әзірбайжан, Tigran Mets 26, Stepanakert, 2600

Ts. Balabekyan

Scientific and Production Center Armbiotechnology NSPO, National Academy of Science

Email: arevik_israelyan@mail.ru
Армения, Gyurjyan 14, Yerevan, 0056

Z. Sargsyan

Center for Epidemiology and Hygiene

Email: arevik_israelyan@mail.ru
Әзірбайжан, Ar. Arakelyan 14, Stepanakert, 2600

F. Tkhruni

Scientific and Production Center Armbiotechnology NSPO, National Academy of Science

Email: arevik_israelyan@mail.ru
Армения, Gyurjyan 14, Yerevan, 0056

Әдебиет тізімі

  1. Aghajanyan A.E., Tkhruni F.N., Hovhannisyan G.S., Eghyan K., Vardanyan A.A., Saghyan A.S. A method of producing and purification of bioinhibitors from culture liquids. Patent RA № 2925 А, 27.04. 2015.
  2. Bauer A.W., Kirby M.M., Sherris J.C., Turk M. Antibiotic susceptibility testing by a standardized single disk method // Am.J. Clin. Pathol. 1966. V. 45. P. 493–496.
  3. Bokulich N., Amiranashvili L., Chitchyan K., Ghazanchyan N., Darbinyan K., Gagelidze N., Sadunishvili T., Goginyan V., Kvesitadze G., Torok T., Mills D. Microbial biogeography of the transnational fermented milk matsoni // Food Microbiology. 2015. P. 12–19. https://doi: 10.1016/j.fm.2015.01.018
  4. Dertli E., Mercan E., Arici M., Yilmaz M.T., Sagdic O. Characterisation of lactic acid bacteria from Turkish sourdough and determination of their exopolysaccharide (EPS) production characteristics // LWT-Food Science and Technology. 2016. V. 71. P. 116–124. https://doi: 10.1016/j.lwt.2016.03.030
  5. Israyelyan A., Karapetyan K., Tkhruni F., Arstamyan L., Balabekyan T. Sensitivity of different pathogens to biological antimicrobial agents // European Journal of Biomedical and Life Sciences. 2015. V. 3. P. 61–66.
  6. Israyelyan A.L., Tkhruni F.N., Arstamyan L.A., Balabekyan T.R., Khachatryan T.V., Karapetyan K.J. Comparative characterization of endemic lactic acid bacteria isolated from several regions of Armenia and Nagorno Karabakh Republics // Biolog. Journ. of Armenia, NAS RA. 2016. P. 50–57.
  7. Israyelyan A.L. Investigation of lactic acid bacteria and their metabiotics isolated from milk of different domestic animals of Artsakh. Dis. Ph D. Yerevan, 2018. P. 116.
  8. Jurášková D., Ribeiro S., Silva C. Exopolysaccharides Produced by Lactic Acid Bacteria: From Biosynthesis to Health-Promoting Properties. Foods. 2022. V. 11. P. 156. https://doi.org/ 10.3390/foods11020156
  9. Karapetyan K., Tkhruni F., Israyelyan A., Yermolenko E., Verdyan A. Comparative characterization of endemic lactic acid bacteria of Enterococcus genus // International Science of technology. 2017. V. 6. P. 357–365.
  10. Parente E., Brienza C., Moles M., Riccardi A. A comparison of methods for measurement of bactriocin activity // Journal of Microbiology Methods. 1995. Vol. 22. P. 95–108.
  11. Tkhruni F., Karapetyan K., Danova S., Dimova S., Karimpur F. Probiotic properties of endemic strains of lactic acid bacteria // J. BioSci. Biotech. 2013. V. 2. P. 1–6.
  12. Tkhruni F., Balabekyan Ts., Karapetyan K., Yakimovich N., Arstamyan L. Antibacterial activity of single cells in the population of lactic acid bacteria // European Journal of Biomedical and Life Sciences. 2015. V. 3. P. 76–80.
  13. Yermolenko E., Chernish A., Aleshina G. Antagonistic activity of Enterococcus faecium L3 against different groups of pathogenic streptococci. Streptococci New Insights Into and Old Enemy. 2006. May. P. 363–366. https://doi.org/10.1016/j.ics.2005.11.030

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2. Fig. 1. Antimicrobial activity of protein-containing fractions obtained after purification of the supernatant of the KZh strain Ent. faecium KE-5 by gel filtration against St. pneumonia

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3. Fig. 2. Effect of antimicrobial drugs against strains isolated from infected eyes, ∅ mm

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4. Fig. 3. Effect of antibiotics on strains isolated from infected eyes, ∅ mm

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5. Fig. 4. Effect of antimicrobial drugs on the growth of bacteria isolated from the pharynx, ∅ mm

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6. Fig. 5. Effect of antibiotics on the growth of isolated bacteria from the throat, ∅ mm

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7. Fig. 6. Antimicrobial activity of BCN 2 (bacteriocin) against some human pathogenic bacteria

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