Cuticle of Hermetia Illucens Black Lion Pupa as A Biosorbent for Probiotics

N. A. Ushakova; Ушакова Н. А.; S. V. Sverguzova; Свергузова С. В.; I. G. Shaikhiev; Шайхиев И. Г.; Zh. A. Sapronova; Сапронова Ж. А.; A. I. Bastrakov; Бастраков А. И.; E. A. Levenko; Левенко Е. А.; R. M. Khatsaeva; Хацаева Р. М.

doi:10.31857/S1026347022601072

Cuticle of Hermetia Illucens Black Lion Pupa as A Biosorbent for Probiotics

Authors: Ushakova N.A.¹, Sverguzova S.V.², Shaikhiev I.G.², Sapronova Z.A.², Bastrakov A.I.¹, Levenko E.A.¹, Khatsaeva R.M.¹
Affiliations:
1. Institute for Problems of Ecology and Evolution, Russian Academy of Sciences, A.N. Severtsov Russian Academy of Sciences
2. Belgorod Technological University. V.G. Shukhov
Issue: No 3 (2023)
Pages: 332-336
Section: КРАТКИЕ СООБЩЕНИЯ
URL: https://rjmseer.com/1026-3470/article/view/647741
DOI: https://doi.org/10.31857/S1026347022601072
EDN: https://elibrary.ru/MOPXXE
ID: 647741

Cite item

Abstract

Using the method of scanning electron microscopy, a comparative study of the particle surface morphology and the elemental composition of the cuticle of Hermetia illucens pupa before and after the application of the culture liquid (CL) of the probiotic strain Lactobacillus acidophilus B 2707 was carried out. At the same time, under the influence of CL, destruction of relatively large particles of the powder of the chitin-protein complex of the cuticle (average particle diameter D 845.3 ± 109.1 μm) and the formation of small fragments (average D 63.4 ± 13.9 μm), including nanoparticles (average D 54 ± 18 nm). A biofilm of lactobacilli enriched with calcium, magnesium and phosphorus was obtained on a sorbent consisting of microparticles of the cuticle of insect pupae and including nanoparticles, which provided the probiotic preparation with high biological efficiency.

Keywords

cuticle, chitin, black soldier fly, sorbent, probiotic

References

Варламов В.П., Ильина А.В., Шагдарова Б.Ц., Луньков А.П., Мысякина И.С. Хитин/хитозан и его производные: фундаментальные и прикладные аспекты // Успехи биол. Хим. 2020. Т. 60. С. 317–368.
Скрябин К.Г., Вихорева Г.А., Варламов В.П. Хитин и хитозан: Получение, свойства и применение. М.: Наука, 2002. 368 с.
Фисинин В.И., Егоров И.А. Руководство по кормлению сельскохозяйственной птицы. Сергиев Посад: ООО “Лика”, 2018. 226 с
Caruso D., Devic E., Subamia IW., Talamond P., Baras E. Technical handbook of domestication and production of diptera Black Soldier Fly (BSF) Hermetia illucens, Stratiomyidae / IRD Editions IPB Press 2014. 141 p. ISBN: 978–979-493-610-8.
Diener S., Zurbrügg C., Tockner K. Conversion of organic material by black soldier fly larvae: establishing optimal feeding rates // Waste Management & Research. 2009. T. 27. № 6. C. 603–610.
Finke M.D. Complete nutrient content of four species of feeder insects // Zoo Biol. 2013. V. 32. № 1. P. 27–36. https://doi.org/10.1002/zoo.21012
Henriques B.S., Garcia E.S., Azambuja P., Ariel F.G. Determination of Chitin Content in Insects: An Alternate Method Based on Calcofluor Staining //Methods article Front. Physiol., Sec. Invertebrate Physiology, 2020. https://doi.org/10.3389/fphys.2020.00117
Lenardon M.D., Munro C.A., Gow N.A. Chitin synthesis and fungal pathogenesis //Current Opinion in Microbiology. 2010. V. 13. P. 416–423.