Polymorphism of the cytochrome b polypeptide of the root vole Alexandromys oeconomus from the North-East Asia and Alaska

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Amino acid sequence polymorphism of cytochrome b enzyme isoforms in 16 samples of Alexandromys oeconomus from the North-East Asia and one sample from Alaska was studied. Forty-three variants of the polypeptide differing among themselves by 33 amino acid substitutions in 30 sites were detected. The distribution of polypeptide isoforms in the samples was determined. The phylogenetic relationships of haplotypes encoding the most common variants of the enzyme were examined. Molecular diversity indices of synonymous haplotypes were determined. Localisation of amino acid substitutions in the spatial configuration of the enzyme was found. The substitutions (nucleotide – T997C and amino acid – F333L), claiming to be genetic markers of the Beringian haplogroup of A. oeconomus, were discovered.

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V. Pereverzeva

Institute of Biological Problems of the North FEB RAS

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Email: vvpereverzeva@mail.ru
俄罗斯联邦, Portovaya st. 18, Magadan, 685000

N. Dokuchaev

Institute of Biological Problems of the North FEB RAS

Email: vvpereverzeva@mail.ru
俄罗斯联邦, Portovaya st. 18, Magadan, 685000

A. Primak

Institute of Biological Problems of the North FEB RAS

Email: vvpereverzeva@mail.ru
俄罗斯联邦, Portovaya st. 18, Magadan, 685000

E. Dubinin

Institute of Biological Problems of the North FEB RAS

Email: vvpereverzeva@mail.ru
俄罗斯联邦, Portovaya st. 18, Magadan, 685000

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2. Fig. 1. Map-scheme of sampling sites for the root vole of the Central Asian and Beringian phylogroups (shown as black and white filled circles, respectively); in case of the presence of both phylogroups, their proportion corresponds to the size of the highlighted sectors. The numbers indicate: 1 – Okhotsk settlement, river basin: 2 – Kulu, 3 – Detrin, 4 – Srednekan; 5 – Evensk settlement, 6 – Omolon river basin, 7 – Chaun, 8 – Paramushir Island, 9 – Kamchatka Peninsula, 10 – Talan Island, 11 – Arman settlement, 12 – Srednekan Island. Misunderstandings, 13 – Magadan, 14 – Ola Lagoon (14a – Ola village, 14b – Atargan village, 14c – Uratamlyan Island), 15 – Fairbanks (Alaska).

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3. Fig. 2. The proportion of cytochrome b enzyme isoforms in root vole samples from Northeast Asia and Alaska.

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4. Fig. 3. Amino acid substitutions in cytochrome b enzyme isoforms in tundra voles of the Central Asian and Beringian phylogroups. Amino acid substitutions are presented relative to the amino acid sequence of the FEcbCA-1 isoform. Substitution sites are shown from the beginning of the polypeptide.

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5. Fig. 4. ML phylogenetic trees constructed using data on the variability of the nucleotide sequence of the cytochrome b gene of the root vole haplotypes of the Central Asian clade encoding the enzyme isoform FEcbCA-1 and the Beringian clade encoding the isoform FEcbBr-1. Bootstrap indices (> 50%) are indicated at branching nodes.

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6. Fig. 5. Haplotypes of the cytochrome b gene FEcbCA-1 – FEcbCA-8 of the root vole. Nucleotide substitutions are presented relative to the nucleotide sequence of the EcbCA-1 haplotype. Substitution sites are shown from the beginning of the cytochrome b gene.

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7. Fig. 6. Haplotypes of the cytochrome b gene of FEcbBr-1 individuals of the root vole. Nucleotide substitutions are presented relative to the nucleotide sequence of the EcbBr-1 haplotype. Substitution sites are shown from the beginning of the cytochrome b gene.

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8. Fig. 7. Sites of amino acid substitutions in the three-dimensional structure of the truncating vole cytochrome b, modeled on the basis of protein homology on the SWISS-MODEL server and visualized in the iCn3D network program.

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