Assessment of the planting material infection and fungal pathogens identification of garlic

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The research results of the main diseases that cause garlic bulb rot in the Moscow region during storage are presented. It was shown that long-term storage (17 months), as expected, increased the rots prevalence and development, both Penicilus: 10.0-12.5%, and Fusarium: 10.0-11.3%. Winter garlic cultivar Nina Arsentyevna’s Memory bulbs by 2 species of Fusarium hade been infected: F. fujikuroi and F. roliferatum identified by PCR analysis and subsequent sequencing of the two isolates target genome. Winter garlic cultivar Pamyati Nina Arsentievna is highly resistant to rots of fungal origin, even after long-term storage (more than a year), in comparison with the reference sample from the Federal Scientific Center for Vegetable Growing, the bulbs of which were completely affected, and corresponded to the fifth defeat point. On winter garlic, the causative agent of penicillium rot, Penicillium glaucum Link, was also found. On collection samples of winter garlic, during selective annual studies, penicillary rot was not found.

About the authors

S. K Temirbekova

Federal State Budgetary Scientific Institution All-Russian Research Institute of Phytopathology

Email: sul20@yandex.ru

O. O Beloshapkina

Russian State Agrarian University - Moscow Agricultural Academy named after K.A. Timiryazev

Yu. V Afanasyeva

Federal Horticultural Center for Breeding, Agrotechnology and Nursery

N. E Ionova

Kazan (Volga region) Federal University

M. M Tareeva

Federal State Budgetary Scientific Institution Federal Scientific Vegetable Center (FSBSI FSVC)

O. B Polivanova

Russian State Agrarian University - Moscow Agricultural Academy named after K.A. Timiryazev

References

  1. Агафонов А.Ф., Герасимова Л.И. Селекция чеснока //Овощеводство. 2007. №8. С. 38-41.
  2. Алексеева Т.В. Усовершенствование способа производства чеснока озимого из воздушных луковичек. Автореф. дисс. … канд. с.-х. наук: 06.01.05, М., 2018.
  3. Ганнибал Ф.Б. Мониторинг альтернариозов сельскохозяйственных культур и идентификация грибов рода Alternaria. Методическое пособие. СПб.: ГНУ ВИЗР Россельхозакадемии, 2011. С. 45-46.
  4. Лазарев А. Вредители лука и чеснока во время вегетации // Овощеводство и тепличное хозяйство. 2011. №1. С. 68-71.
  5. Пивоваров В.Ф., Никульшин В.П., Тимина Л.Т., Шестакова К.С. Патогенная микрофлора чеснока озимого //Вестник РАСХН. 2009. №5. С. 63-64.
  6. Поляков А.В. Важнейшие вопросы развития чесноководства в Российской Федерации // Экологические проблемы современного овощеводства и качество овощной продукции: сб. науч. тр. М.: ФГБНУ ВНИИО, 2014. Вып. 1. С. 436-442.
  7. Скорина В.В., Кохтенкова И.Г. Сравнительная оценка коллекционных сортообразцов чеснока озимого по урожайности. Овощи России. 2021. №3. С. 60-67. DOI: https: // doi.org / 10.18619 / 2072-9146-2021-3-60-67.
  8. Тимина Л.Т., Енгалычева И.А. Комплекс патогенов на овощных культурах в условиях центрального региона РФ // Овощи России. 2015. № 3-4. С. 123-129.
  9. Тимина Л.Т., Шестакова К.С., Никульшин В.П. Влияние аллицина на устойчивость сортообразцов чеснока озимого // Селекция и семеноводство овощных культур. М.: Изд-во ВНИИССОК. 2015. Вып. 46. С. 561-568.
  10. Филюшин М.А., Данилова О.А., Середин Т.М. Идентификация патогенных грибов в луковицах чеснока при хранении и в корневой сфере в период роста растений //Овощи России. 2021. №3. С. 105-109.
  11. Чекмарев В.В. Гриб Fusarium proliferatum и его чувствительность к современным фунгицидам // Agricultural sciences / "Colloquium-journal". 2020. 18(70). С.-28-332.
  12. Шестакова К.С., Никульшин В.П., Тимина Л.Т. Методика заражения и оценка устойчивости чеснока к фузариозной гнили // Аграрный вестник Урала. 2011. № 4(83). С.78-79.
  13. Ali S., Ganai B.A., Kamili A.N. et al. Pathogenesis-related proteins and peptides as promising tools for engineering plants with multiple stress tolerance. Microbiol. Res. 2018;212-213:29-37.
  14. Ali S., Ganai B.A., Kamili A.N. et al. Pathogenesis-related proteins and peptides as promising tools for engineering plants with multiple stress tolerance. Microbiol. Res. 2018;212-213:29-37.
  15. Anisimova, M.A., Kochieva O.K., Shchennikova E.Z., Genome-Wide A.V. Identification and Expression of Chitinase Class I Genes in Garlic (Allium sativum L.) Cultivars Resistant and Susceptible to Fusarium proliferatum. Plants. 2021;10:720.
  16. Bai S., Dong C., Li B., Dai H. A PR-4 gene identified from Malus domestica is involved in the defense responses against Botryosphaeria dothidea. Plant Physiol. Biochem. 2013;62:23-32.
  17. Bharti P., Jyoti P., Kapoor P. et al. Host-Induced Silencing of Pathogenicity Genes Enhances Resistance to Fusarium oxysporum Wilt in Tomato. Mol. Biotechnol. 2017;59:343-352.
  18. Chen J., Piao Y., Liu Y. et al. Genome-wide identification and expression analysis of chitinase gene family in Brassica rapa reveals its role in clubroot resistance. Plant Sci. 2018;270:257-267.
  19. Chodorska M., Paduch-Cichal E., Kalinowska E., Szyndel M.S. Assessment of allexiviruses infection in garlic plants in poland //Acta Sci. Pol., Hortorum Cultus, 2014. V. 13(2). P. 179-186.
  20. De Jesús-Pires C., Ferreira-Neto J.R.C., Pacifico Bezerra-Neto J. et al. Plant Thaumatin-like Proteins: Function, Evolution and Biotechnological Applications. Curr. Protein Pept. Sci. 2020;21:36-51.
  21. Gagkaeva,T., Gavrilova O., Orina A. et al. Analysis of Toxigenic Fusarium Species Associated with Wheat Grain from Three Regions of Russia: Volga, Ural, and West Siberia. Toxins. 2019; 11:252.
  22. Gálvez L., Urbaniak M., Waśkiewicz A. et al. Fusarium proliferatum - Causal agent of garlic bulb rot in Spain: Genetic variability and mycotoxin production. Food Microbiol. 2017;67:41-48.
  23. Gao Y., Zan X., Wu X. et al. Identification of Fungus-Responsive Cis-Acting Element in the Promoter of Brassica Juncea Chitinase Gene, BjCHI1. Plant Sci. 2014;215-216:190-198.
  24. Kalman B., Abraham D., Graph S. et al. Isolation and Identification of Fusarium spp., the Causal Agents of Onion (Allium cepa) Basal Rot in Northeastern Israel. Biology. 2020;9:69.
  25. Kumar S., Stecher G., Tamura K. MEGA7: Molecular evolutionary genetics analysis version 7.0. Molecular biology and evolution. Mol. Biol. Evol. 2016;33:1870-1874.
  26. Kumar S.; Stecher G., Li M. et al. MEGA X: Molecular Evolutionary Genetics Analysis across Computing Platforms. //Mol. Biol. Evol. 2018. № 35. Р.1547-1549.
  27. Leyronas C., Chrétien P.L., Troulet C. et al. First report of Fusarium proliferatum causing garlic clove rot in France. Plant Dis. 2018; 102:2658.
  28. Liu S., Kracher B., Ziegler J. et al. Negative regulation of ABA signaling by WRKY33 is critical for Arabidopsis immunity towards Botrytis cinerea 2100. eLife. 2015;4:e07295.
  29. Li G., Zhou J., Jia H. et al. Mutation of a histidine-rich calcium-binding-protein gene in wheat confers resistance to Fusarium head blight. Nat. Genet. 2019;51:1106-1112.
  30. Mondani L., Chiusa G., Battilani P. Fungi associated with garlic during the cropping season, with focus on Fusarium proliferatum and F. oxysporum. // Plant Health Progress. 2021.
  31. Samet M., Charfeddine M., Kamoun L. et al. Effect of compost tea containing phosphogypsum on potato plant growth and protection against Fusarium solani infection. Environ. Sci. Pollut. Res. Int. 2018;25:18921-18937.
  32. Sun X., Zhu S., Li N. et al. A Chromosome-Level Genome Assembly of Garlic (Allium sativum) Provides Insights into Genome Evolution and Allicin Biosynthesis. Mol. Plant. 2020;13:1328-1339.
  33. Tan R., Collins P.J., Wang J. et al. Different loci associated with root and foliar resistance to sudden death syndrome (Fusarium virguliforme) in soybean. Theor. Appl. Genet. 2019;132:501-513.
  34. Verma V., Ravindran P., Kumar P.P. Plant hormone-mediated regulation of stress responses. BMC Plant Biol. 2016;16:86.
  35. Yang J., Duan G., Li C. et al. The Crosstalks Between Jasmonic Acid and Other Plant Hormone Signaling Highlight the Involvement of Jasmonic Acid as a Core Component in Plant Response to Biotic and Abiotic Stresses. Front. Plant Sci. 2019;10:1349.

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