Expression of Hox cluster genes in arly larval development of sea urchin Strongylocentrotus intermedius (Echinodermata, Echinoidea)

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Abstract

The quantitative assessment of the expression of 11 genes of the Hox cluster of the sea urchin Strongylocentrotus intermedius (SiHox1, 2, 3, 5, 6, 7, 8, 9/10, 11/ 13a, 11/13b, 11/13c) in the larvae at early developmental stages (blastula – 13 hours post fertilization (h.p.f.), gastrula – 35 h.p.f., prism stage – 46 h.p.f., echinoplutei – 4 and 9 days p.f.) was carried out. At the blastula stage, the expression of the SiHox-7, 11/13b, and 11/13c is observed; as for the latter the early activation was noted for the first time for regular sea urchins. At the gastrula and prism stages, a very low level of expression is observed. At the pluteus stage, there is significant increase of the expression of all Hox cluster genes. Breakages of temporal colinearity during the expression of Hox genes in echinoderms are discussed.

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About the authors

N. V. Ageenko

A.V. Zhirmunsky National Scientific Center of Marine Biology, Far Eastern Branch of the Russian Academy of Sciences (NSCMB FEB RAS)

Email: olga_ejova@mail.ru
Russian Federation, Vladivostok

O. V. Ezhova

Lomonosov Moscow State University

Author for correspondence.
Email: olga_ejova@mail.ru

Биологический факультет

Russian Federation, Moscow

K. V. Kiselev

Federal Scientific Center of the East Asia Terrestrial Biodiversity, Far Eastern Branch of the Russian Academy of Sciences

Email: olga_ejova@mail.ru
Russian Federation, Vladivostok

A. I. Lukinykh

Lomonosov Moscow State University

Email: olga_ejova@mail.ru

Биологический факультет

Russian Federation, Moscow

V. V. Malakhov

Lomonosov Moscow State University

Email: olga_ejova@mail.ru

Academician of the RAS

Russian Federation, Moscow

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2. Fig. 1. Early developmental stages of Strongylocentrotus intermedius larvae taken for the study: A – blastula, 13 h.p.o., B – gastrula, 35 h.p.o., C – prism stage, 46 h.p.o., G – echinopluteus, 4 days h.p.o., D – echinopluteus, 9 days h.p.o. Scale: 50 µm.

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3. Fig. 2. Results of quantitative assessment of the expression of Hox-cluster genes of Strongylocentrotus intermedius at early stages of larval development (in relative units – r.u.).

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