In vitro and in vivo biodegradation of silk fabric scaffolds

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Abstract

This study investigates the biodegradation of natural silk scaffolds made from gauze and satin fabrics under in vitro and in vivo conditions. Experiments were conducted using phosphate-buffered saline and Fenton’s reagent to model degradation. The samples demonstrated high stability under physiological conditions’ model and exhibited varying degradation rates under oxidative stress. In vivo studies on rats revealed good biocompatibility of the scaffolds and a gradual reduction in inflammatory responses. These findings highlight the potential of silk scaffolds for use in various areas of regenerative medicine.

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About the authors

I. I. Agapov

Academician V.I. Shumakov Federal Research Center of Transplantology and Artificial Organs, Ministry of Health of the Russian Federation

Author for correspondence.
Email: igor.agapov@gmail.com
Russian Federation, Moscow

E. I. Podbolotova

Academician V.I. Shumakov Federal Research Center of Transplantology and Artificial Organs, Ministry of Health of the Russian Federation; Moscow Institute of Physics and Technology

Email: igor.agapov@gmail.com
Russian Federation, Moscow; Dolgoprudny

E. A. Nemets

Academician V.I. Shumakov Federal Research Center of Transplantology and Artificial Organs, Ministry of Health of the Russian Federation

Email: igor.agapov@gmail.com
Russian Federation, Moscow

L. A. Kirsanova

Academician V.I. Shumakov Federal Research Center of Transplantology and Artificial Organs, Ministry of Health of the Russian Federation

Email: igor.agapov@gmail.com
Russian Federation, Moscow

N. V. Grudinin

Academician V.I. Shumakov Federal Research Center of Transplantology and Artificial Organs, Ministry of Health of the Russian Federation

Email: igor.agapov@gmail.com
Russian Federation, Moscow

A. R. Pashutin

Academician V.I. Shumakov Federal Research Center of Transplantology and Artificial Organs, Ministry of Health of the Russian Federation; Moscow Institute of Physics and Technology

Email: igor.agapov@gmail.com
Russian Federation, Moscow; Dolgoprudny

O. I. Agapova

Academician V.I. Shumakov Federal Research Center of Transplantology and Artificial Organs, Ministry of Health of the Russian Federation

Email: igor.agapov@gmail.com
Russian Federation, Moscow

A. E. Efimov

Academician V.I. Shumakov Federal Research Center of Transplantology and Artificial Organs, Ministry of Health of the Russian Federation

Email: igor.agapov@gmail.com
Russian Federation, Moscow

Yu. B. Basok

Academician V.I. Shumakov Federal Research Center of Transplantology and Artificial Organs, Ministry of Health of the Russian Federation

Email: igor.agapov@gmail.com
Russian Federation, Moscow

A. V. Lyundup

Рeoples’ Friendship University of Russia

Email: igor.agapov@gmail.com
Russian Federation, Moscow

S. V. Gautier

Academician V.I. Shumakov Federal Research Center of Transplantology and Artificial Organs, Ministry of Health of the Russian Federation; Sechenov University

Email: igor.agapov@gmail.com

Academician of the RAS

Russian Federation, Moscow; Moscow

References

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2. Fig. 1. Samples obtained during the study (5×): A – satin, G – gas; the numbers indicate the degree of material processing: 0 and 80%, respectively.

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3. Fig. 2. Histological picture of samples on days 4, 14 and 56 of implantation (200×). Hematoxylin and eosin staining. a – A80 on day 4, b – A80 on day 14, c – A80 on day 56, d – G80 on day 4, d – G80 on day 14, e – G80 on day 56.

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