SUPER-SENSITIVE REGISTRATION OF POLYFUNCTIONAL MAGNETIC NANOMATERIALS FOR THE RAPID DETERMINATION OF MOLECULAR MARKERS OF DISEASES

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Abstract

Magnetic particles (MPs) based on superparamagnetic iron oxide nanocrystals are increasingly used in modern research for the development of express technologies for high-precision determination of the concentration of biomolecules. In this work, we developed a universal method for using MPs as polyfunctional agents that perform several different functions in one analysis for topical issues of biochemical and medical diagnostics, based on the original technology of ultrasensitive detection of nonlinear magnetic nanomaterials. The method was tested for the rapid quantitative registration of cardiac markers and SARS-CoV-2-associated molecular markers in complex matrices. The proposed method is promising for creating test systems for prehospital monitoring, particularly for emergency molecular diagnostics in patients with suspected acute myocardial infarction, as well as for the rapid detection of coronavirus infection.

About the authors

A. V. Orlov

Prokhorov General Physics Institute of the Russian Academy of Sciences

Author for correspondence.
Email: alexey.orlov@kapella.gpi.ru
Russia, Moscow

Yu. A. Malkerov

Prokhorov General Physics Institute of the Russian Academy of Sciences; National Research Nuclear University MEPhI

Email: petr.nikitin@nsc.gpi.ru
Russia, Moscow; Russia, Moscow

A. M. Skirda

Prokhorov General Physics Institute of the Russian Academy of Sciences; National Research Nuclear University MEPhI

Email: petr.nikitin@nsc.gpi.ru
Russia, Moscow; Russia, Moscow

D. O. Novichikhin

Prokhorov General Physics Institute of the Russian Academy of Sciences; National Research Nuclear University MEPhI

Email: petr.nikitin@nsc.gpi.ru
Russia, Moscow; Russia, Moscow

S. L. Znoyko

Prokhorov General Physics Institute of the Russian Academy of Sciences

Email: petr.nikitin@nsc.gpi.ru
Russia, Moscow

V. A. Bragina

Prokhorov General Physics Institute of the Russian Academy of Sciences

Email: petr.nikitin@nsc.gpi.ru
Russia, Moscow

P. I. Nikitin

Prokhorov General Physics Institute of the Russian Academy of Sciences

Author for correspondence.
Email: petr.nikitin@nsc.gpi.ru
Russia, Moscow

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Copyright (c) 2023 А.В. Орлов, Ю.А. Малкеров, А.М. Скирда, Д.О. Новичихин, С.Л. Знойко, В.А. Брагина, П.И. Никитин