Cholecalciferol Exhibits no Antibacterial Effect on Staphylococcus aureus and Escherichia coli: An in vitro Study


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Abstract

Background::The pleiotropic effect of cholecalciferol (vitamin D3) has gained significant momentum and has been explored widely.

Objectives::The study aimed to investigate the antimicrobial effect of cholecalciferol against S. aureus and E. coli.

Methods::An in-vitro study was performed for the antimicrobial effect of cholecalciferol against S. aureus and E. coli. The minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) were determined following the broth microdilution method.

Results::The MIC value of cholecalciferol against both S. aureus and E. coli was 0.312 mg/ml, and the MBC for both organisms was 1.25 mg/ml. However, we also observed a significant antimicrobial effect in the dimethyl sulfoxide (DMSO) control at 12.5% (v/v). Therefore, the observed antimicrobial effect may be attributed to DMSO, indicating cholecalciferol does not directly inhibit S. aureus and E. coli.

Conclusion::This study indicates that cholecalciferol does not directly inhibit S. aureus and E. coli. Hence, we suggest exploring the antibacterial properties of other vitamin D analogs, such as calcitriol or its synergetic effect with other antimicrobial agents.

About the authors

Shilia Kurian

Department of Pharmacy Practice, Manipal College of Pharmaceutical Sciences, Manipal Academy of High Education

Email: info@benthamscience.net

Avirup Biswas

Department of Pharmaceutical Biotechnology, Manipal College of Pharmaceutical Sciences, Manipal Academy of High Education

Email: info@benthamscience.net

Shrivathsa Kulavalli

Department of Pharmacy Practice, Manipal College of Pharmaceutical Sciences, Manipal Academy of High Education

Email: info@benthamscience.net

Jesil Aranjani

Department of Pharmaceutical Biotechnology, Manipal College of Pharmaceutical Sciences, Manipal Academy of High Education

Email: info@benthamscience.net

Amrita Pattanaik

Manipal Institute of Virology, Manipal Academy of High Education

Email: info@benthamscience.net

Murali Munisamy

Department of Translational Medicine, All India Institute of Medical Sciences

Email: info@benthamscience.net

Kavitha Saravu

Manipal Centre for Infectious Diseases, Prasanna School of Public Health, Manipal Academy of Higher Education

Email: info@benthamscience.net

Gabriel Rodrigues

Consultant General and Laparoscopic Surgeon, Aster Al Raffah Hospital

Email: info@benthamscience.net

Sonal Miraj

Department of Pharmacy Practice, Manipal College of Pharmaceutical Sciences, Manipal Academy of High Education

Author for correspondence.
Email: info@benthamscience.net

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