Study of EA.hy926 Endothelial Cells by Atomic Force and Scanning Ion-Conductance Microscopy

S. N. Pleskova; Плескова С. Н.; N. A. Bezrukov; Безруков Н. А.; E. N. Gorshkova; Горшкова Е. Н.; S. Z. Bobyk; Бобык С. З.; E. V. Lazarenko; Лазаренко Е. В.

doi:10.31857/S0041377123050073

Study of EA.hy926 Endothelial Cells by Atomic Force and Scanning Ion-Conductance Microscopy

Authors: Pleskova S.N.¹^,2, Bezrukov N.A.¹, Gorshkova E.N.¹, Bobyk S.Z.¹, Lazarenko E.V.¹^,2
Affiliations:
1. Lobachevsky State University of Nizhny Novgorod, Scanning Probe Microscopy Laboratory
2. Nizhny Novgorod State Technical University, Department of Nanotechnology and Biotechnology
Issue: Vol 65, No 5 (2023)
Pages: 437-446
Section: Articles
URL: https://rjmseer.com/0041-3771/article/view/669626
DOI: https://doi.org/10.31857/S0041377123050073
EDN: https://elibrary.ru/GZFQWG
ID: 669626

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Abstract

A two-section analytical system was developed and tested to study the culture of EA.hy926 endothelial cells in real time with high resolution imaging. Scanning ion-conductance microscopy was shown as more relevant method because it didn’t cause mechanical damage of cell, and made possible scanning on the membranes, when endothelial cells were surrounded by nutrient medium. The method allowed not only to analyze changes in the cells morphology, but also to identify extracellular (microfilaments) and intracellular (nucleolus) structures. The rigidity mapping showed that the rigidity of the endotheliocyte membrane varied from 357 to 796 Pa. After 240 min from the beginning of the observation, the formation of endothelial cells apoptotic bodies has begun, and the rigidity of the cell gradually increased, while rigidity of the apoptotic bodies decreased.

Keywords

endotheliocytes, apoptosis, membrane rigidity, cell morphology, microstructures, two-section analytical chamber, scanning ion-conductance microscopy, atomic force microscopy

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